Evaluasi Aktivitas Antioksidan Ekstrak Metanol Daun Kutere (Teijsmanniodendron hollrungii) Asal Papua Barat Menggunakan Metode DPPH

Muhammad Fajar Islam; Sitti Fatimah Ramadhani; Sabir Sumarna; Jamius bin Stephanus

doi:10.55681/armada.v4i10.4672

Authors

Muhammad Fajar Islam Kimia, Fakultas Matematika dan Ilmu Alam, Universitas Papua, Indonesia
Sitti Fatimah Ramadhani Pendidikan Kimia, Fakultas Keguruan dan Ilmu Pendidikan, Universitasa Papua, Indonesia
Sabir Sumarna Kimia, Fakultas Matematika dan Ilmu Alam, Universitas Papua, Indonesia
Jamius bin Stephanus Kimia, Fakultas Matematika dan Ilmu Alam, Universitas Papua, Indonesia

DOI:

https://doi.org/10.55681/armada.v4i10.4672

Keywords:

Teijsmaniadendron Holrungii, Antioksidan, DPPH, Papua, Ekstrak Metanol

Abstract

Papua memiliki keanekaragaman tumbuhan yang berpotensi sebagai sumber senyawa bioaktif, tetapi informasi mengenai aktivitas antioksidan daun kutere (Teijsmanniodendron hollrungii) masih terbatas. Penelitian ini bertujuan mengevaluasi aktivitas antioksidan ekstrak metanol daun T. hollrungii melalui pengujian penangkapan radikal bebas. Penelitian menggunakan metode eksperimental laboratorium dengan ekstraksi maserasi terhadap 40,4 g sampel daun menggunakan 3 L metanol selama 3 × 24 jam. Aktivitas antioksidan diuji menggunakan metode 2,2-difenil-1-pikrilhidrazil (DPPH) pada konsentrasi ekstrak 5, 6, 7, 8, dan 9 ppm. Peneliti mengukur absorbansi menggunakan spektrofotometer UV-Vis pada panjang gelombang 517 nm dan menganalisis persentase inhibisi serta nilai IC₅₀ melalui regresi linear. Hasil penelitian menunjukkan rendemen ekstrak sebesar 5,85% dengan persentase inhibisi yang meningkat dari 5,315% menjadi 8,607%. Analisis regresi menghasilkan estimasi IC₅₀ sebesar 60,58 ppm, tetapi nilai tersebut masih memerlukan konfirmasi karena berada di luar rentang konsentrasi pengujian. Penelitian ini memberikan bukti awal aktivitas penangkapan radikal DPPH oleh ekstrak daun T. hollrungii dan menjadi dasar pengembangan penelitian fitokimia serta evaluasi antioksidan lebih lanjut.

Downloads

Download data is not yet available.

References

Budiarty, M.. Maruzy, A., Mujahid, R., Sari, AN., Jokopriyambodo, W., Widayat, T., Wahyono, S. 2020., The use of Antimalarial Plants as Traditional Treatment in Papua Island, Indonesia. Heliyon: vol 6 2020.

Cámara-Leret, R., Frodin, D. G., Adema, F., Anderson, C., Appelhans, M. S., Argent, G., Arias Guerrero, S., Ashton, P., Baker, W. J., Barfod, A. S., Barrington, D., Borosova, R., Bramley, G. L. C., Briggs, M., Buerki, S., Cahen, D., Callmander, M. W., Cheek, M., Chen, C. W., … van Welzen, P. C. (2020). New Guinea has the world’s richest island flora. Nature, 584(7822), 579–583.

Cámara-Leret, R., Frodin, D. G., Adema, F., et al. (2020). New Guinea has the world's richest island flora. Nature, 584(7822), 579–583. https://doi.org/10.1038/s41586-020-2549-5

Choi, J., Whang W.K., Kim. H.J.,2004. Studies on Anti-imflammatory effect of Clerodendrum trichotomum Thunberg leaves. Archieves of pharmaceutical Research vol 27 no.2 page 189-193, 2004.

de Kok, R. P. J., Rusea, G., & Latiff, A. (2009). The genus Teijsmanniodendron Koord. (Lamiaceae). Kew Bulletin, 64, 587–625. https://doi.org/10.1007/s12225-009-9156-9

de Menezes, B. B., Frescura, L. M., Duarte, R., Villetti, M. A., & da Rosa, M. B. (2021). A critical examination of the DPPH method: Mistakes and inconsistencies in stoichiometry and IC₅₀ determination by UV–Vis spectroscopy. Analytica Chimica Acta, 1157, Article 338398. https://doi.org/10.1016/j.aca.2021.338398

Gülçin, İ., & Alwasel, S. H. (2023). DPPH radical scavenging assay. Processes, 11(8), Article 2248. https://doi.org/10.3390/pr11082248

Kar, P., Goyal A.K., Das, A.P., Sen, A. 2014. Antioxidant and Pharmaceutical Potential of Clerodendrum L.; An overview. International Journal of Green Pharmacy, October 2014.

Khound, P., Sarma, H., Sarma, P. P., et al. (2023). Ultrasound-assisted extraction of verbascoside from Clerodendrum glandulosum leaves for analysis of antioxidant and antidiabetic activities. ACS Omega, 8(23), 20360–20369. https://doi.org/10.1021/acsomega.3c00173

Lang, Y., Gao, N., Zang, Z., Meng, X., Lin, Y., Yang, S., Yang, Y., Jin, Z., & Li, B. (2024). Classification and antioxidant assays of polyphenols: A review. Journal of Future Foods, 4(3), 193–204. https://doi.org/10.1016/j.jfutfo.2023.07.002

Lense, O. 2012. The Wild Plansts used as Traditional Medicines by Indigenous People of Manokwari, West Papua. Biodiversitas: Volume 13 No.2, April 2012.

Limsakul, S., Monthakantirat, O., Chulikhit, Y., Maneenet, J., Khamphukdee, C., Chotritthirong, Y., Phasomsap, A., Boonyarat, C., & Daodee, S. (2024). Optimizing extraction, evaluating antioxidant activity, and analyzing bioactive compounds in Trikaysornmas formula. Advances in Pharmacological and Pharmaceutical Sciences, 2024(1), Article 8335536. https://doi.org/10.1155/2024/8335536

Mamuaja, M. N., Herlina, T., Rumampuk, R. J., Maksum, I. P., & Rukayadi, Y. (2024). Flavonoids from Clerodendrum genus and their biological activities. ADMET & DMPK, 12(6), 843–879. https://doi.org/10.5599/admet.2442

Maruzy, A dan Mujahid R. 2019. Status Konservasi Tumbuhan Obat Provinsi Papua dan Papua Barat. Media Konservasi: Vol 24 No.2 (2019).

Maturbongs, A. C., Sianipar, F. R. D. N., & Serio, R. (2024). Eksplorasi dan skrining fitokimia tumbuhan obat tradisional masyarakat asli Papua di Desa Sairo dan Desa Lebau Kabupaten Manokwari. Jurnal Biogenerasi, 9(1), 758–765. https://doi.org/10.30605/biogenerasi.v9i1.3477

Moshari-Nasirkandi, A., Alirezalu, A., Alipour, H., & Amato, J. (2023). Screening of 20 species from Lamiaceae family based on phytochemical analysis, antioxidant activity and HPLC profiling. Scientific Reports, 13(1), Article 16987. https://doi.org/10.1038/s41598-023-44337-7

Ndhlala, A. R., Işık, M., Yüksel, A. K., & Dikici, E. (2024). Phenolic content analysis of two species belonging to the Lamiaceae family: Antioxidant, anticholinergic, and antibacterial activities. Molecules, 29(2), Article 480. https://doi.org/10.3390/molecules29020480

Nwachukwu, I. D., Sarteshnizi, R. A., Udenigwe, C. C., & Aluko, R. E. (2021). A concise review of current in vitro chemical and cell-based antioxidant assay methods. Molecules, 26(16), Article 4865. https://doi.org/10.3390/molecules26164865

Parsch, C., Wagner, B., Adam, MP., Nitscke C., Kreft, H., Schrader, J.,2022. Papua at the Crossroad: A Plea for Systematic Conservation Planning in One Of the Largest Remaining Areas of Tropical Rainforest. Perspetive: In forest and global change.

Siburian, R. H., & Angrianto, R. (2024). Utilization of plants as traditional medicine by the people of Momiwaren Village, West Papua. Median: Jurnal Ilmu Ilmu Eksakta, 16(1), 1–10. https://doi.org/10.33506/md.v16i1.3209

Spréa, R. M., Caleja, C., Finimundy, T. C., Calhelha, R. C., Pires, T. C. S. P., Amaral, J. S., Prieto, M. A., Ferreira, I. C. F. R., Pereira, E., & Barros, L. (2024). Chemical and bioactive evaluation of essential oils from edible and aromatic Mediterranean Lamiaceae plants. Molecules, 29(12), Article 2827. https://doi.org/10.3390/molecules29122827

Sumarna, S., Islam, MF., Irunsah, A., Sadsoetoeboen, MJ., Mandatjan, KI., 2023. Phytochemical Screening and Antibacterial Activity of Teijsmaniadendron holrungii from West Papua. Jurnal techno vol. 12 No. 1 Mei 2022.

Tristantini, D., Ismawati A., Pradana BT., Jonathan JG., 2016. Pengujian Aktivitas Antioksidan Menggunakan metode DPPH pada daun Tanjung (Mimusops elengi L). Prosiding Seminar Nasional Teknik Kimia; Maret 2016.

Tukayo, B. L. A., & Samalo, R. (2023). Inventory of medicinal plants the treatment and prevention of malaria in the East Sentani District, Jayapura Regency. Fitofarmaka: Jurnal Ilmiah Farmasi, 13(1), 9–19. https://doi.org/10.33751/jf.v13i1.6396

Yamauchi, M., Kitamura, Y., Nagano, H., Kawatsu, J., & Gotoh, H. (2024). DPPH measurements and structure–activity relationship studies on the antioxidant capacity of phenols. Antioxidants, 13(3), Article 309. https://doi.org/10.3390/antiox13030309

Zamljen, T., Grohar, M. C., & Medic, A. (2024). Mint-scented species in Lamiaceae: An abundant and varied reservoir of phenolic and volatile compounds. Foods, 13(12), Article 1857. https://doi.org/10.3390/foods13121857